Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Changes in microvascular density differentiate metabolic health outcomes in monkeys with prior radiation exposure and subsequent skeletal muscle ECM remodeling

doi: 10.1152/ajpregu.00108.2017

Figure Lengend Snippet: Heat shock protein (HSP) 90 associates with endothelial nitric oxide synthase (eNOS) to improve its function. A: HSP90 levels were suppressed by radiation eposure (P = 0.01 for Non-Rad-CTL vs. Rad-CTL and RAD-DM), which were most reduced in T2DM monkeys (P = 0.03 for Non-Rad-CTL and Rad-CTL vs. Rad-DM). Values are means ± SE; n = 8 in each group. Different superscript letters (a and b) denote significant difference between groups (P < 0.05). B and C: HSP90 significantly correlated with plasma nitrate (NO3) and tumor necrosis factor-related ligand 1A (TNFR1A).

Article Snippet: Heat shock protein (HSP) 90 (HSP90) in muscle homogenate was quantified in duplicate using a sandwich ELISA with the HSP90 Alpha ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) according to the manufacturer’s instructions.

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Changes in microvascular density differentiate metabolic health outcomes in monkeys with prior radiation exposure and subsequent skeletal muscle ECM remodeling

doi: 10.1152/ajpregu.00108.2017

Figure Lengend Snippet: A and B: greater fibrotic signaling, as indicated by TGFβ levels in skeletal muscle, correlated with less-abundant HSP90 and vascular endothelial growth factor (VEGF). C and D: increasing abundance of TGFβ1 and resultant collagen IV deposition in skeletal muscle were associated with greater muscle lipid, as measured by muscle triglyceride (TG) content.

Article Snippet: Heat shock protein (HSP) 90 (HSP90) in muscle homogenate was quantified in duplicate using a sandwich ELISA with the HSP90 Alpha ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) according to the manufacturer’s instructions.

Techniques:

Journal: Journal of Integrative Agriculture

Article Title: Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek's Diseases

doi: 10.1016/s2095-3119(13)60514-6

Figure Lengend Snippet: Fig. 1 Representative photomicrographs of myocardium tissues. A, tumor cells (arrow) were observed in the myocardial fibers of chickens infected with MD (H.E. staining). B, positive immunolabelling of Hsp90 (arrow) was localized mainly in the cytoplasm of tumor cells (IHC staining). C, Hsp90 positive signals in negative control chickens (IHC staining).

Article Snippet: The Hsp90 concentration in the tissues was determined according to the manufacturer’s protocol (Cat. no. CSBE12873c, Chicken heat shock protein 90 (Hsp90) ELISA Kit, Cusabio Biotech Co., Ltd., China).

Techniques: Infection, Staining, Immunohistochemistry, Negative Control

Journal: Journal of Integrative Agriculture

Article Title: Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek's Diseases

doi: 10.1016/s2095-3119(13)60514-6

Figure Lengend Snippet: Fig. 3 Representative photomicrographs of kidney tissues. A, tumor cells (arrow) were observed in the kidney tissues of chickens infected with MD (H.E. staining). B, positive immunolabelling of Hsp90 (arrow) was localized mainly in the cytoplasm of tumor cells (IHC staining). C, Hsp90 positive signals in negative control chickens (IHC staining).

Article Snippet: The Hsp90 concentration in the tissues was determined according to the manufacturer’s protocol (Cat. no. CSBE12873c, Chicken heat shock protein 90 (Hsp90) ELISA Kit, Cusabio Biotech Co., Ltd., China).

Techniques: Infection, Staining, Immunohistochemistry, Negative Control

Journal: Journal of Integrative Agriculture

Article Title: Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek's Diseases

doi: 10.1016/s2095-3119(13)60514-6

Figure Lengend Snippet: Fig. 2 Representative photomicrographs of liver tissues. A, tumor cells (arrow) were observed in the liver tissues of chickens infected with MD (H.E. staining). B, positive immunolabelling of Hsp90 (arrow) was localized mainly in the cytoplasm of tumor cells (IHC staining). C, Hsp90 positive signals in negative control chickens (IHC staining).

Article Snippet: The Hsp90 concentration in the tissues was determined according to the manufacturer’s protocol (Cat. no. CSBE12873c, Chicken heat shock protein 90 (Hsp90) ELISA Kit, Cusabio Biotech Co., Ltd., China).

Techniques: Infection, Staining, Immunohistochemistry, Negative Control

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Changes in microvascular density differentiate metabolic health outcomes in monkeys with prior radiation exposure and subsequent skeletal muscle ECM remodeling

doi: 10.1152/ajpregu.00108.2017

Figure Lengend Snippet: Heat shock protein (HSP) 90 associates with endothelial nitric oxide synthase (eNOS) to improve its function. A: HSP90 levels were suppressed by radiation eposure (P = 0.01 for Non-Rad-CTL vs. Rad-CTL and RAD-DM), which were most reduced in T2DM monkeys (P = 0.03 for Non-Rad-CTL and Rad-CTL vs. Rad-DM). Values are means ± SE; n = 8 in each group. Different superscript letters (a and b) denote significant difference between groups (P < 0.05). B and C: HSP90 significantly correlated with plasma nitrate (NO3) and tumor necrosis factor-related ligand 1A (TNFR1A).

Article Snippet: Heat shock protein (HSP) 90 (HSP90) in muscle homogenate was quantified in duplicate using a sandwich ELISA with the HSP90 Alpha ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) according to the manufacturer’s instructions.

Techniques:

Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

Article Title: Changes in microvascular density differentiate metabolic health outcomes in monkeys with prior radiation exposure and subsequent skeletal muscle ECM remodeling

doi: 10.1152/ajpregu.00108.2017

Figure Lengend Snippet: A and B: greater fibrotic signaling, as indicated by TGFβ levels in skeletal muscle, correlated with less-abundant HSP90 and vascular endothelial growth factor (VEGF). C and D: increasing abundance of TGFβ1 and resultant collagen IV deposition in skeletal muscle were associated with greater muscle lipid, as measured by muscle triglyceride (TG) content.

Article Snippet: Heat shock protein (HSP) 90 (HSP90) in muscle homogenate was quantified in duplicate using a sandwich ELISA with the HSP90 Alpha ELISA kit (StressMarq Biosciences, Victoria, BC, Canada) according to the manufacturer’s instructions.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Blestriarene C exerts an inhibitory effect on triple-negative breast cancer through multiple signaling pathways

doi: 10.3389/fphar.2024.1434812

Figure Lengend Snippet: BC induced apoptosis in BT549 cells. (A) Cell viability of BT549 cells after combination with Z-VAD-FMK. (B) Results of Annexin V-FITC/PI apoptosis assay after BC treatment. (C) Images of apoptosis-related protein expression bands. (D) Statistical results of apoptosis-related protein expression. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 versus control group).

Article Snippet: Annexin V-FITC/PI Apoptosis Detection Kit, the primary antibodies against HSP90AA1, PTGS2, and GAPDH were purchased from BOSTER (California, United States).

Techniques: Apoptosis Assay, Expressing, Control